Enzymes for Life Sciences
Thermolabile dsDNase I
Thermolabile double strand DNase I is an endonuclease that cleaves phosphodiester bonds in DNA to release oligonucleotides with 5´-phosphorylated and 3´-hydroxylated ends. Thermolabile dsDNase I has a particularly strong preference for double-stranded DNA. In the presence of Mg2+, as the only divalent cation, and using oligos as substrate, the activity towards ssDNA is minimum than towards dsDNA and can be easily inactivate by heat treatment (55 °C). It can be used to specifically degrade dsDNA, leaving ssDNA and RNA intact.
Catalog
Reference | Unit size | Concentration | Format | Special characteristics | Associated files |
MT01U-G1DNATXA | 1,000 U | 2 U/µL | Liquid, glycerol-free | N/A** | |
MT01U-G1DNATXA-RF | 1,000 U | 2 U/µL | Liquid, glycerol-free | RNase-free | |
MT01U-L1DNATXA | 1,000 U | N/A** | Lyophilized | N/A** | |
MT01U- S1DNATXA | 1,000 U | 2 U/µL | Liquid, in glycerol | N/A** | |
MT01U- S1DNATXA-RF | 1,000 U | 2 U/µL | Liquid, glycerol-free | RNase-free |
*Please, do not hesitate to contact us for further requirements.
**Not applicable
Applications
- Genomic DNA removal from RNA samples prior to RT-PCR
- DNA template digestion following in vitro transcription
- Preparation of DNA-free samples
According to the purpose, this product is available in its RNase-free version.
*For research use only.
Suggested reaction conditions
Recommended use of 2 U of thermolabile dsDNase I per 50 μL of sample or per 0.2-1 μg RNA in provided buffer (Ref: BDNATXA), incubated at 37 °C during 30 minutes for total DNA digestion. Thermolabile dsDNase I can be inactivated at 55 °C for 15 minutes.
Activity unit definition
One Unit of activity is defined as an increase in absorbance at 260 nm of 0.001 per minute at 25 °C on the assay conditions (50 μg/mL calf thymus DNA in buffer 20 mM Tris, 10 mM MgCl2, pH 7.5).
Check the Technical Data Sheet for more information.
Storage conditions
Once received, store the product at -80 °C o -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature.
Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C.
Buffer composition
Liquid products are commercialized with 10X Thermolabile dsDNase I reaction buffer, while the lyophilized product only contains the enzyme.
Product:
-
- 01U-G1DNATXA: 20 mM Tris pH 7.5, 10 mM MgCl2 (also commercialized as RNase-free product with the following product code: 01U-G1DNATXA-RF)
- 01U-S1DNATXA: 20 mM Tris pH 7.5, 10 mM MgCl2 50% glycerol (also commercialized as RNase-free product with the following product code: 01U-S1DNATXA-RF)
Buffer:
-
- BDNATXA (10X concentrated): 200 mM Tris pH 7.5, 100 mM MgCl2
Recommended resuspension buffer for the lyophilized products:
-
- For long-term storage: 20 mM Tris pH 7.5, 10 mM MgCl2, 50% glycerol
- For short-term storage: 20 mM Tris pH 7.5, 10 mM MgCl2
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