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Enzymes for Life Sciences

Thermolabile dsDNase I

Thermolabile double strand DNase I is an endonuclease that cleaves phosphodiester bonds in DNA to release oligonucleotides with 5´-phosphorylated and 3´-hydroxylated ends. Thermolabile dsDNase I has a particularly strong preference for double-stranded DNA. In the presence of Mg2+, as the only divalent cation, and using oligos as substrate, the activity towards ssDNA is minimum than towards dsDNA and can be easily inactivate by heat treatment (55 °C). It can be used to specifically degrade dsDNA, leaving ssDNA and RNA intact. 

Catalog

Reference 

Unit size  

Concentration 

Format 

Special characteristics 

Associated files 

MT01U-G1DNATXA 

1,000 U 

2 U/µL 

Liquid, glycerol-free 

N/A** 

TDS 

SDS 

MT01U-G1DNATXA-RF 

1,000 U 

2 U/µL 

Liquid, glycerol-free 

RNase-free 

TDS 

SDS 

MT01U-L1DNATXA 

1,000 U 

N/A** 

Lyophilized  

N/A** 

TDS 

SDS 

MT01U- S1DNATXA 

1,000 U 

2 U/µL 

Liquid, in glycerol 

N/A** 

TDS 

SDS 

MT01U- S1DNATXA-RF 

1,000 U 

2 U/µL 

Liquid, glycerol-free 

RNase-free 

TDS 

SDS 

*Please, do not hesitate to contact us for further requirements.  

**Not applicable 

Applications

According to the purpose, this product is available in its RNase-free version.
*For research use only. 

Suggested reaction conditions

Recommended use of 2 U of thermolabile dsDNase I per 50 μL of sample or per 0.2-1 μg RNA in provided buffer (Ref: BDNATXA), incubated at 37 °C during 30 minutes for total DNA digestion. Thermolabile dsDNase I can be inactivated at 55 °C for 15 minutes.

Activity unit definition

One Unit of activity is defined as an increase in absorbance at 260 nm of 0.001 per minute at 25 °C on the assay conditions (50 μg/mL calf thymus DNA in buffer 20 mM Tris, 10 mM MgCl2, pH 7.5). 

Check the Technical Data Sheet for more information.

Storage conditions

Once received, store the product at -80 °C o -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature.  

Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C. 

Buffer composition

Liquid products are commercialized with 10X Thermolabile dsDNase I reaction buffer, while the lyophilized product only contains the enzyme. 

Product: 
    • 01U-G1DNATXA: 20 mM Tris pH 7.5, 10 mM MgCl2 (also commercialized as RNase-free product with the following product code: 01U-G1DNATXA-RF) 
    • 01U-S1DNATXA: 20 mM Tris pH 7.5, 10 mM MgCl2 50% glycerol (also commercialized as RNase-free product with the following product code: 01U-S1DNATXA-RF) 
Buffer: 
    • BDNATXA (10X concentrated): 200 mM Tris pH 7.5, 100 mM MgCl2  
 Recommended resuspension buffer for the lyophilized products: 
    • For long-term storage: 20 mM Tris pH 7.5, 10 mM MgCl2, 50% glycerol 
    • For short-term storage: 20 mM Tris pH 7.5, 10 mM MgCl2

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