PCR Enzymes and Solutions
qPCR MasterMix (MasterYeast®) for DNA and RNA samples
MasterMix is a mixture of PCR reagents for DNA or RNA amplification to be used in conventional or real time PCR, which is very fast and precise. MasterYeast® contains Taq Hot Start DNA polymerase, dNTPs, MgCl2 and buffer. For RNA amplification, MasterYeast® also contains Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT). MasterYeast® for DNA or RNA samples are available with or without a green dye.
MasterYeast® for DNA samples
MasterYeast® for DNA samples is a 2X concentrated Hot Start PCR Master Mix, optimized for DNA amplification in conventional PCR and real-time PCR, both in monoplex and multiplex. This product provides a flexible tool that simplifies the PCR workflow and allows the amplification of DNA samples in a wide range of amplicon size (50 bp to 2 kb). MasterYeast® contains a Hot Start Taq DNA polymerase, increasing the specificity of the DNA amplification. MasterYeast® is formed by a solution of Hot Start Taq DNA polymerase, dNTPs and an optimized buffer. This product is available with or without a green dye. The user only has to add the DNA sample, primers and, if necessary, probe.
Catalog
Applications
- High-throughput PCR
- Routine PCR with lower risk of contamination and pipetting errors
- qPCR
Since this Taq DNA polymerase is expressed in yeast, it does not have E. coli DNA contamination, which makes this product more suitable for bacterial DNA analysis than other Taq DNA polymerases expressed in E. coli.
*For research use only.
Suggested reaction conditions
Recommended use of 1X MasterYeast® and primers, probe, if necessary, and variable amount of DNA sample must be added. DNA MasterYeast® can be used in common PCR conditions.
**Please, see the technical data sheet for more information.
Activity unit definition
One unit of activity is defined as the amount of enzyme needed to incorporate 10 nmol of dNTPs (deoxyribonucleotides) into a DNA fragment, in 30 minutes at 72 °C.
Storage conditions
Once received, store the product at -80 °C o -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature. Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C.
MasterYeast® for RNA samples
Catalog
*For research use only
Reference | Size | State | Probe | Associated files |
MT05R-SRMYeast | 500 reactions | Liquid, in glycerol | No | Coming soon. |
MT05R-LRMYeast | 500 reactions | Lyophilized | No | Coming soon. |
MT05R-SRMYeast-Green | 500 reactions | Liquid, in glycerol | Green-dye | Coming soon. |
MT05R-LRMYeast-Green | 500 reactions | Lyophilized | Green-dye | Coming soon. |
*Please, do not hesitate to contact us for further requirements.
Applications
- Amplification of RNA
- Detection of mRNA expression levels
Since this M-MLV Reverse Transcriptase and Taq DNA polymerase are expressed in yeast, they do not have E. coli DNA contamination, which make this product more suitable for bacterial DNA analysis than other M-MLV Reverse Transcriptase and Taq DNA polymerase expressed in E. coli.
*For research use only.
Suggested reaction conditions
Ongoing…
Activity unit definition
Ongoing…
Storage conditions
Ongoing…
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PCR Enzymes and solutions
Polymerase chain reaction (PCR) is one of the most common techniques in molecular biology, having a wide range of applications. Conventional PCR is a qualitative technique for detection and amplification of nucleic acids. However, real time PCR, also known as quantitative PCR (qPCR), allows sensitive and specific quantification of nucleic acids. The product portfolio of Levprot includes enzymes for conventional PCR and real time PCR, but also for reverse-transcription PCR, DNA digestion and ligation reactions, as well as Master Mixes for DNA and RNA samples.
mRNA Synthesis
mRNA synthesis has gained importance since mRNA vaccines and therapeutics became relevant in biopharma. Synthesis of mRNA requires several steps from DNA template to pure mRNA, including post-translational modifications and additional components needed for mRNA synthesis. Levprot offers a product portfolio that includes products that covers all of the above.
Isotermal amplification
Isothermal nucleic acid amplification is a unique method to amplify limited amounts of DNA at constant temperature, eliminating the thermal cycling process and thus, the need of a thermocycler equipment. One of the most common isothermal amplification methods is LAMP (loop-mediated isothermal amplification), in which loop structures facilitate rounds of amplification using 4-6 primers. At Levprot we offer a Bst DNA polymerase which is uniquely useful for a variety of isothermal amplification reactions.
CLIA Enzymes
Chemiluminescent immunoassay (CLIA) is a technique that uses luminescent molecules which emit electromagnetic radiation (i.e. producing light) triggered by a chemical reaction. This immunoassay technique is more sensitive than conventional colorimetric ELISA methods as it allows the detection of target molecules in biological samples at zeptomole scale (10-21 mol) due to chemiluminescent signal multiplication and amplification.
Blocking buffers
Blocking buffers are inert solutions of a single or a combination of proteins that bind onto surfaces of different nature. These buffers prevent non-specific interactions of antibodies or antigens to membranes or plates, reducing background interferences and increasing the signal-to-noise ratio in immunoassays.
Next generation Sequencing
Next Generation Sequencing (NGS) is a parallel sequencing tech that offers more speed and scalability and is very useful to determine the order of nucleotides in entire genomes of DNA or RNA. NGS has several enzymatic workflow steps, in which polymerases, restriction enzymes, ligases and kinases-phosphorilases have an important role.
Antigens
Antigens are foreign molecules that trigger an immune response acting against what is considered a possible external attack. Their presence makes the immune system produce antibodies against them. The production of antigens is useful for immunoassays as they allow the specific detection of antibodies (i.e. markers of disease) against an antigen.
