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PCR Enzymes and Solutions

qPCR MasterMix (MasterYeast®) for DNA and RNA samples

MasterMix is a mixture of PCR reagents for DNA or RNA amplification to be used in conventional or real time PCR, which is very fast and precise. MasterYeast® contains Taq Hot Start DNA polymerase, dNTPs, MgCl2 and buffer. For RNA amplification, MasterYeast® also contains Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT).

MasterYeast® for DNA samples

MasterYeast® for DNA samples is a 2X concentrated Hot Start PCR Master Mix, optimized for DNA amplification in conventional PCR and real-time PCR, both in monoplex and multiplex. This product provides a flexible tool that simplifies the PCR workflow and allows the amplification of DNA samples in a wide range of amplicon size (50 bp to 2 kb). MasterYeast® contains a Hot Start Taq DNA polymerase, increasing the specificity of the DNA amplification. MasterYeast® is formed by a solution of Hot Start Taq DNA polymerase, dNTPs and an optimized buffer. This product is available with or without a green dye. The user only has to add the DNA sample, primers and, if necessary, probe.

Catalog

Cat. No.

Size

Format

Probe

Associated files

MT05R-SDMYeast-Green

500 reactions

Liquid, in glycerol

Green-dye

TDS

SDS

MT05R-SDMYeast

5 x 100 reactions

Liquid, in glycerol

None

TDS

SDS

MT01R-SDMYeast

100 reactions

Liquid, in glycerol

None

TDS

SDS

MT05R-LDMYeast

10 x 50 reactions

Lyophilized

None

TDS

SDS

MT01R-LDMYeast

2 x 50 reactions

Lyophilized

None

TDS

SDS

MT05R-SDMYeast-Green

Size

500 reactions

Format

Liquid, in glycerol

Probe

Green-dye

Associated Files

TDSSDS

MT05R-SDMYeast

Size

5 x 100 reactions

Format

Liquid, in glycerol

Probe

None

Associated Files

TDSSDS

MT01R-SDMYeast

Size

100 reactions

Format

Liquid, in glycerol

Probe

None

Associated Files

TDSSDS

MT05R-LDMYeast

Size

10 x 50 reactions

Format

Liophilized

Probe

None

Associated Files

TDSSDS

MT01R-LDMYeast

Size

2 x 50 reactions

Format

Lyophilized

Probe

None

Associated Files

TDS SDS

Applications

Since this Taq DNA polymerase is expressed in yeast, it does not have E. coli DNA contamination, which makes this product more suitable for bacterial DNA analysis than other Taq DNA polymerases expressed in E. coli.

 
*For research use only.

Suggested reaction conditions

Recommended use of 1X MasterYeast® and primers, probe, if necessary, and variable amount of DNA sample must be added. DNA MasterYeast® can be used in common PCR conditions.

**Please, see the technical data sheet for more information.

Activity unit definition

One unit of activity is defined as the amount of enzyme needed to incorporate 10 nmol of dNTPs (deoxyribonucleotides) into a DNA fragment, in 30 minutes at 72 °C.

Storage conditions

Once received, store the product at -80 °C o -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature. Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C.

MasterYeast® for RNA samples

Catalog

*For research use only

ReferenceSize StateProbeAssociated files
MT05R-SRMYeast5 x 100 reactionsLiquid, in glycerolNone

TDS

SDS

MT01R-SRMYeast100 reactionsLiquid, in glycerolNone

TDS

SDS

MT05R-LRMYeast10 x 50 reactionsLyophilizedNone

TDS

SDS

MT01R-LRMYeast2 x 50 reactionsLyophilizedNone

TDS

SDS

*Please, do not hesitate to contact us for further requirements.

MT05R-SRMYeast

Size

5 x 100 reactions

Format

Liquid, in glycerol

Probe

None

Associated Files

TDS | SDS

MT01R-SRMYeast

Size

100 reactions

Format

Liquid, in glycerol

Probe

None

Associated Files

TDSSDS

MT05R-LRMYeast

Size

10 x 50 reactions

Format

Lyophilized

Probe

None

Associated Files

TDSSDS

MT01R-LRMYeast

Size

10 x 50 reactions

Format

Lyophilized

Probe

None

Associated Files

TDS | SDS

MT01R-LDMYeast

Size

2 x 50 reactions

Format

Lyophilized

Probe

None

Associated Files

 

TDS | SDS

Applications

Since this M-MLV Reverse Transcriptase and Taq DNA polymerase are expressed in yeast, they do not have E. coli DNA contamination, which make this product more suitable for bacterial DNA analysis than other M-MLV Reverse Transcriptase and Taq DNA polymerase expressed in E. coli.

*For research use only. 

Suggested reaction conditions

Ongoing…

Activity unit definition

Ongoing…

Storage conditions

Ongoing…

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