Enzymes for Life Sciences
DNase I from Bos taurus
DNase I is an endonuclease from Bos taurus that non-specifically cleaves DNA to release di-, tri- and oligonucleotide products with 5’-phosphorylated and 3’-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.
Catalog
| Cat. No. | Unit size | Concentration | Format | Special characteristics | Associated files |
| MT01U-L1DNAIHA | 1,000 U | N/A** | Lyophilized | N/A** | |
| MT01U-S1DNAIHA | 1,000 U | 1 U/µL | Liquid, in glycerol | N/A** | |
| MT01U- S1DNAIHA-RF | 1,000 U | 1 U/µL | Liquid, in glycerol | RNase-free |
*Please, do not hesitate to contact us for further requirements.
**Not applicable
Applications
- Genomic DNA removal from RNA samples prior to RT-PCR
- DNA template digestion following in vitro transcription
- Preparation of DNA-free samples
According to the purpose, this product is available in its RNase-free version.
*For research use only.
Suggested reaction conditions
Recommended use of 2 U of DNase I from Bos taurus per 50 μL of sample or per 0.2-1 μg RNA in provided buffer (Ref.: BDNAIHA), incubated at 37 °C during 30 min for total DNA digestion. DNase I from Bos taurus can be inactivated by adding EDTA and incubating at 75 °C for 10 min.
*Please,see the technical data sheet for more information.
Activity unit definition
One Unit of activity is defined as an increase in absorbance at 260 nm of 0.001 per minute at 25 °C on the assay conditions (50 μg/mL calf thymus DNA in buffer 20 mM Tris, 10 mM MgCl2, 0.1 mM CaCl2, pH 7.5).
Storage conditions
Once received, store the product at -80 °C o -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature.
Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C.
Buffer composition
Liquid products are commercialized with 10X DNase I from Bos taurus reaction buffer, while the lyophilized product only contains the enzyme.
Product:
- 01U-G1DNAIHA: 20 mM Tris pH 7.5, 10 mM MgCl2, 0.1 mM CaCl2 (also commercialized as RNase-free product with the following product code: 01U-G1DNAIHA-RF)
- 01U-S1DNAIHA: 20 mM Tris pH 7.5, 10 mM MgCl2, 0.1 mM CaCl2, 50% glycerol (also commercialized as RNase-free product with the following product code: 01U-G1DNAIHA-RF)
Buffer:
- BDNAIHA (10X concentrated): 100 mM Tris pH 7.5, 100 mM MgCl2, 1 mM CaCl2
Recommended resuspension buffer for the lyophilized products:
- For long-term storage: 20 mM Tris pH 7.5, 10 mM MgCl2, 0.1 mM CaCl2, 50% glycerol
- For short-term storage: 20 mM Tris pH 7.5, 10 mM MgCl2, 0.1 mM CaCl2
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