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Enzymes for Life Sciences

DNase I from Bos taurus

DNase I is an endonuclease from Bos taurus that non-specifically cleaves DNA to release di-, tri- and oligonucleotide products with 5’-phosphorylated and 3’-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids. 

Catalog

Reference  Unit size   Concentration  Format  Special characteristics  Associated files 
MT10U-L1DNAIHA  10,000 U  N/A**  Lyophilized   N/A**  TDS SDS
MT10U- S1DNAIHA  10,000 U  10 U/µL  Liquid, in glycerol  N/A**  TDS SDS
MT10U- S1DNAIHA-RF  10,000 U  10 U/µL  Liquid, in glycerol  RNase-free  TDS SDS
*Please, do not hesitate to contact us for further requirements.   **Not applicable

Applications

According to the purpose, this product is available in its RNase-free version.

*For research use only. 

Suggested reaction conditions

Recommended use of 2 U of DNase I from Bos taurus per 50 μL of sample or per 0.2-1 μg RNA in provided buffer (Ref.: BDNAIHA), incubated at 37 °C during 30 min for total DNA digestion. DNase I from Bos taurus can be inactivated by adding EDTA and incubating at 75 °C for 10 min.  

*Please,see the technical data sheet for more information. 

Activity unit definition

One Unit of activity is defined as an increase in absorbance at 260 nm of 0.001 per minute at 25 °C on the assay conditions (50 μg/mL calf thymus DNA in buffer 20 mM Tris, 10 mM MgCl2, 0.1 mM CaCl2, pH 7.5). 

Storage conditions

Once received, store the product at -80 °C o -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature.  

Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C. 

Buffer composition

Liquid products are commercialized with 10X DNase I from Bos taurus reaction buffer, while the lyophilized product only contains the enzyme. 

Product: 
    • 01U-G1DNAIHA: 20 mM Tris pH 7.5, 10 mM MgCl2, 0.1 mM CaCl2 (also commercialized as RNase-free product with the following product code: 01U-G1DNAIHA-RF) 
    • 01U-S1DNAIHA: 20 mM Tris pH 7.5, 10 mM MgCl2, 0.1 mM CaCl2, 50% glycerol (also commercialized as RNase-free product with the following product code: 01U-G1DNAIHA-RF) 
 Buffer: 
    • BDNAIHA (10X concentrated): 100 mM Tris pH 7.5, 100 mM MgCl2, 1 mM CaCl2 

 

Recommended resuspension buffer for the lyophilized products: 
    • For long-term storage: 20 mM Tris pH 7.5, 10 mM MgCl2, 0.1 mM CaCl2, 50% glycerol 
    •  For short-term storage: 20 mM Tris pH 7.5, 10 mM MgCl2, 0.1 mM CaCl2 

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