Cerrar este cuadro de búsqueda.

Enzymes for Life Sciences

DNase I from Bos taurus

DNase I is an endonuclease from Bos taurus that non-specifically cleaves DNA to release di-, tri- and oligonucleotide products with 5’-phosphorylated and 3’-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids. 


Reference  Unit size   Concentration  Format  Special characteristics  Associated files 
MT10U-L1DNAIHA  10,000 U  N/A**  Lyophilized   N/A**  TDS SDS
MT10U- S1DNAIHA  10,000 U  10 U/µL  Liquid, in glycerol  N/A**  TDS SDS
MT10U- S1DNAIHA-RF  10,000 U  10 U/µL  Liquid, in glycerol  RNase-free  TDS SDS
*Please, do not hesitate to contact us for further requirements.   **Not applicable


According to the purpose, this product is available in its RNase-free version.

*For research use only. 

Suggested reaction conditions

Recommended use of 2 U of DNase I from Bos taurus per 50 μL of sample or per 0.2-1 μg RNA in provided buffer (Ref.: BDNAIHA), incubated at 37 °C during 30 min for total DNA digestion. DNase I from Bos taurus can be inactivated by adding EDTA and incubating at 75 °C for 10 min.  

*Please,see the technical data sheet for more information. 

Activity unit definition

One Unit of activity is defined as an increase in absorbance at 260 nm of 0.001 per minute at 25 °C on the assay conditions (50 μg/mL calf thymus DNA in buffer 20 mM Tris, 10 mM MgCl2, 0.1 mM CaCl2, pH 7.5). 

Storage conditions

Once received, store the product at -80 °C o -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature.  

Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C. 

Buffer composition

Liquid products are commercialized with 10X DNase I from Bos taurus reaction buffer, while the lyophilized product only contains the enzyme. 

    • 01U-G1DNAIHA: 20 mM Tris pH 7.5, 10 mM MgCl2, 0.1 mM CaCl2 (also commercialized as RNase-free product with the following product code: 01U-G1DNAIHA-RF) 
    • 01U-S1DNAIHA: 20 mM Tris pH 7.5, 10 mM MgCl2, 0.1 mM CaCl2, 50% glycerol (also commercialized as RNase-free product with the following product code: 01U-G1DNAIHA-RF) 
    • BDNAIHA (10X concentrated): 100 mM Tris pH 7.5, 100 mM MgCl2, 1 mM CaCl2 


Recommended resuspension buffer for the lyophilized products: 
    • For long-term storage: 20 mM Tris pH 7.5, 10 mM MgCl2, 0.1 mM CaCl2, 50% glycerol 
    •  For short-term storage: 20 mM Tris pH 7.5, 10 mM MgCl2, 0.1 mM CaCl2 

More Products

Other recombinant proteins you can buy

PCR Enzymes and solutions

Polymerase chain reaction (PCR) is one of the most common techniques in molecular biology, having a wide range of applications. Conventional PCR is a qualitative technique for detection and amplification of nucleic acids. However, real time PCR, also known as quantitative PCR (qPCR), allows sensitive and specific quantification of nucleic acids. The product portfolio of Levprot includes enzymes for conventional PCR and real time PCR, but also for reverse-transcription PCR, DNA digestion and ligation reactions, as well as Master Mixes for DNA and RNA samples.

mRNA Synthesis

mRNA synthesis has gained importance since mRNA vaccines and therapeutics became relevant in biopharma. Synthesis of mRNA requires several steps from DNA template to pure mRNA, including post-translational modifications and additional components needed for mRNA synthesis. Levprot offers a product portfolio that includes products that covers all of the above.

Isotermal amplification

Isothermal nucleic acid amplification is a unique method to amplify limited amounts of DNA at constant temperature, eliminating the thermal cycling process and thus, the need of a thermocycler equipment. One of the most common isothermal amplification methods is LAMP (loop-mediated isothermal amplification), in which loop structures facilitate rounds of amplification using 4-6 primers. At Levprot we offer a Bst DNA polymerase which is uniquely useful for a variety of isothermal amplification reactions.

CLIA Enzymes

Chemiluminescent immunoassay (CLIA) is a technique that uses luminescent molecules which  emit electromagnetic radiation (i.e. producing light) triggered by a chemical reaction. This immunoassay technique is more sensitive than conventional colorimetric ELISA methods as it allows the detection of target molecules in biological samples at zeptomole scale (10-21 mol) due to chemiluminescent signal multiplication and amplification.

Blocking buffers

Blocking buffers are inert solutions of a single or a combination of proteins that bind onto surfaces of different nature. These buffers prevent non-specific interactions of antibodies or antigens to membranes or plates, reducing background interferences and increasing the signal-to-noise ratio in immunoassays.

Next generation Sequencing

Next Generation Sequencing (NGS) is a parallel sequencing tech that offers more speed and scalability and is very useful to determine the order of nucleotides in entire genomes of DNA or RNA. NGS has several enzymatic workflow steps, in which polymerases, restriction enzymes, ligases and kinases-phosphorilases have an important role.


Antigens are foreign molecules that trigger an immune response acting against what is considered a possible external attack. Their presence makes the immune system produce antibodies against them. The production of antigens is useful for immunoassays as they allow the specific detection of antibodies (i.e. markers of disease) against an antigen.

Enzymes for life sciences

Explore our range of enzymes and specialized products designed to enhance your experiments in molecular biology. A variety of essential enzymes, including DNases, Proteinase K and recombinant BSA, we provide everything you need to optimize your laboratory and ensure precise and reliable results in your research.