mRNA Protein Synthesis
Inorganic pyrophosphatase 1
Inorganic pyrophosphatase 1 (iPP1) catalyzes the hydrolysis of inorganic pyrophosphate to two orthophosphates. iPP1 is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is highly specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if Zn2+ is present. iPP1 has an homodimeric complex, consisting in two equal 32 kDa subunits.
Catalog
Reference | Unit size | Concentration | Format | Associated files |
MT01U-G1IPPHDA | 100 U | 0.1 U/µL | Liquid, glycerol-free | |
MT01U- S1IPPHDA | 100 U | 0.1 U/µL | Liquid, in glycerol | |
MT01U-L1IPPHDA | 100 U | N/A** | Lyophilized | TDS SDS |
*Please, do not hesitate to contact us for further requirements.
**Not applicable
Applications
- High yield RNA synthesis by in vitro transcription
- Enhancement of DNA replication: preventing accumulation of pyrophosphate
According to the purpose, this product is available in its RNase-free version.
*For research use only.
Suggested reaction conditions
Recommended use of 1-3 units per mL of sample in buffer 100 mM Tris pH 7.5, 1-10 mM MgCl2 and 2 mM inorganic pyrophosphatase (iPP) in a high-yield in vitro RNA synthesis reaction.
Liquid versions of this product are provided in buffer 100X concentrated.
Activity unit definition
One unit of the enzyme hydrolysis 1 µmol of inorganic pyrophosphate in 1 min at 25 °C. Enzyme activity is assayed in the following mixture: 100 mM Tris pH 7.5, 2 mM MgCl2 and 2 mM inorganic pyrophosphate (PPi).
Storage conditions
Once received, store the product at -80 °C or -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature.
Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -20 °C or -80 °C.
Buffer composition
Liquid products are commercialized with 10X Inorganic Pyrophosphatase I reaction buffer, while the lyophilized product only contains the enzyme.
Product:
- 01U-G1IPPHDA: 20 mM Tris pH 7,5, 100 mM NaCl, 0,1 mM EDTA, 1 mM DTT
- 01U-S1IPPHDA: 20 mM Tris pH 7.5, 100 mM NaCl, 50% glycerol, 0.1 mM EDTA, 1 mM DTT
Buffer:
- B1IPPHDA (10X concentrated): 1 M Tris pH 7.5, 20 mM MgCl2
Recommended resuspension buffer for the lyophilized products:
- For long-term storage: 20 mM Tris pH 7.5, 100 mM NaCl, 50% glycerol, 0.1 mM EDTA, 1 mM DTT
- For short-term storage: 20 mM Tris pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT
More Products
Other recombinant proteins you can buy
PCR Enzymes and solutions
Polymerase chain reaction (PCR) is one of the most common techniques in molecular biology, having a wide range of applications. Conventional PCR is a qualitative technique for detection and amplification of nucleic acids. However, real time PCR, also known as quantitative PCR (qPCR), allows sensitive and specific quantification of nucleic acids. The product portfolio of Levprot includes enzymes for conventional PCR and real time PCR, but also for reverse-transcription PCR, DNA digestion and ligation reactions, as well as Master Mixes for DNA and RNA samples.
mRNA Synthesis
mRNA synthesis has gained importance since mRNA vaccines and therapeutics became relevant in biopharma. Synthesis of mRNA requires several steps from DNA template to pure mRNA, including post-translational modifications and additional components needed for mRNA synthesis. Levprot offers a product portfolio that includes products that covers all of the above.
Isotermal amplification
Isothermal nucleic acid amplification is a unique method to amplify limited amounts of DNA at constant temperature, eliminating the thermal cycling process and thus, the need of a thermocycler equipment. One of the most common isothermal amplification methods is LAMP (loop-mediated isothermal amplification), in which loop structures facilitate rounds of amplification using 4-6 primers. At Levprot we offer a Bst DNA polymerase which is uniquely useful for a variety of isothermal amplification reactions.
CLIA Enzymes
Chemiluminescent immunoassay (CLIA) is a technique that uses luminescent molecules which emit electromagnetic radiation (i.e. producing light) triggered by a chemical reaction. This immunoassay technique is more sensitive than conventional colorimetric ELISA methods as it allows the detection of target molecules in biological samples at zeptomole scale (10-21 mol) due to chemiluminescent signal multiplication and amplification.
Blocking buffers
Blocking buffers are inert solutions of a single or a combination of proteins that bind onto surfaces of different nature. These buffers prevent non-specific interactions of antibodies or antigens to membranes or plates, reducing background interferences and increasing the signal-to-noise ratio in immunoassays.
Next generation Sequencing
Next Generation Sequencing (NGS) is a parallel sequencing tech that offers more speed and scalability and is very useful to determine the order of nucleotides in entire genomes of DNA or RNA. NGS has several enzymatic workflow steps, in which polymerases, restriction enzymes, ligases and kinases-phosphorilases have an important role.
Antigens
Antigens are foreign molecules that trigger an immune response acting against what is considered a possible external attack. Their presence makes the immune system produce antibodies against them. The production of antigens is useful for immunoassays as they allow the specific detection of antibodies (i.e. markers of disease) against an antigen.
