mRNA Protein Synthesis

Inorganic pyrophosphatase 1

Inorganic pyrophosphatase 1 (iPP1) catalyzes the hydrolysis of inorganic pyrophosphate to two orthophosphates. iPP1 is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is highly specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if Zn2+ is present. iPP1 has an homodimeric complex, consisting in two equal 32 kDa subunits. 


Reference Unit size  Concentration Format Associated files 
MT01U-G1IPPHDA 100 U 0.1 U/µL Liquid, glycerol-free 



MT01U- S1IPPHDA 100 U 0.1 U/µL Liquid, in glycerol 



*Please, do not hesitate to contact us for further requirements.  

**Not applicable 


Unit size  



Associated files 


100 U 

0.1 U/µL 

Liquid, glycerol-free 




100 U 

0.1 U/µL 

Liquid, in glycerol 




100 U 





*Please, do not hesitate to contact us for further requirements.  

**Not applicable 


According to the purpose, this product is available in its RNase-free version.
*For research use only. 

Suggested reaction conditions

Recommended use of 1-3 units per mL of sample in buffer 100 mM Tris pH 7.5, 1-10 mM MgCl2 and 2 mM inorganic pyrophosphatase (iPP) in a high-yield in vitro RNA synthesis reaction.  

Liquid versions of this product are provided in buffer 100X concentrated.  

Activity unit definition

One unit of the enzyme hydrolysis 1 µmol of inorganic pyrophosphate in 1 min at 25 °C. Enzyme activity is assayed in the following mixture: 100 mM Tris pH 7.5, 2 mM MgCl2 and 2 mM inorganic pyrophosphate (PPi). 

Storage conditions

Once received, store the product at -80 °C or -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature.  

Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -20 °C or -80 °C. 

Buffer composition

Liquid products are commercialized with 10X Inorganic Pyrophosphatase I reaction buffer, while the lyophilized product only contains the enzyme. 

    • 01U-G1IPPHDA: 20 mM Tris pH 7,5, 100 mM NaCl, 0,1 mM EDTA, 1 mM DTT  
    • 01U-S1IPPHDA: 20 mM Tris pH 7.5, 100 mM NaCl, 50% glycerol, 0.1 mM EDTA, 1 mM DTT  
    • B1IPPHDA (10X concentrated): 1 M Tris pH 7.5, 20 mM MgCl2 
Recommended resuspension buffer for the lyophilized products: 
    • For long-term storage: 20 mM Tris pH 7.5, 100 mM NaCl, 50% glycerol, 0.1 mM EDTA, 1 mM DTT  
    • For short-term storage: 20 mM Tris pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT 

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