mRNA Protein Synthesis

Inorganic pyrophosphatase 1

Inorganic pyrophosphatase 1 (iPP1) catalyzes the hydrolysis of inorganic pyrophosphate to two orthophosphates. iPP1 is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is highly specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if Zn2+ is present. iPP1 has an homodimeric complex, consisting in two equal 32 kDa subunits. 

Catalog

Reference Unit size  Concentration Format Associated files 
MT01U-G1IPPHDA 100 U 0.1 U/µL Liquid, glycerol-free 

TDS

SDS

MT01U- S1IPPHDA 100 U 0.1 U/µL Liquid, in glycerol 

TDS

SDS

*Please, do not hesitate to contact us for further requirements.  

**Not applicable 

Reference 

Unit size  

Concentration 

Format 

Associated files 

MT01U-G1IPPHDA 

100 U 

0.1 U/µL 

Liquid, glycerol-free 

TDS

SDS

MT01U- S1IPPHDA 

100 U 

0.1 U/µL 

Liquid, in glycerol 

TDS

SDS

MT01U-L1IPPHDA 

100 U 

N/A** 

Lyophilized  

TDS 

SDS 

*Please, do not hesitate to contact us for further requirements.  

**Not applicable 

Applications

According to the purpose, this product is available in its RNase-free version.
*For research use only. 

Suggested reaction conditions

Recommended use of 1-3 units per mL of sample in buffer 100 mM Tris pH 7.5, 1-10 mM MgCl2 and 2 mM inorganic pyrophosphatase (iPP) in a high-yield in vitro RNA synthesis reaction.  

Liquid versions of this product are provided in buffer 100X concentrated.  

Activity unit definition

One unit of the enzyme hydrolysis 1 µmol of inorganic pyrophosphate in 1 min at 25 °C. Enzyme activity is assayed in the following mixture: 100 mM Tris pH 7.5, 2 mM MgCl2 and 2 mM inorganic pyrophosphate (PPi). 

Storage conditions

Once received, store the product at -80 °C or -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature.  

Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -20 °C or -80 °C. 

Buffer composition

Liquid products are commercialized with 10X Inorganic Pyrophosphatase I reaction buffer, while the lyophilized product only contains the enzyme. 

Product: 
    • 01U-G1IPPHDA: 20 mM Tris pH 7,5, 100 mM NaCl, 0,1 mM EDTA, 1 mM DTT  
    • 01U-S1IPPHDA: 20 mM Tris pH 7.5, 100 mM NaCl, 50% glycerol, 0.1 mM EDTA, 1 mM DTT  
Buffer: 
    • B1IPPHDA (10X concentrated): 1 M Tris pH 7.5, 20 mM MgCl2 
Recommended resuspension buffer for the lyophilized products: 
    • For long-term storage: 20 mM Tris pH 7.5, 100 mM NaCl, 50% glycerol, 0.1 mM EDTA, 1 mM DTT  
    • For short-term storage: 20 mM Tris pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT 

More Products

Other recombinant proteins you can buy

PCR Enzymes and solutions

Polymerase chain reaction (PCR) is one of the most common techniques in molecular biology, having a wide range of applications. Conventional PCR is a qualitative technique for detection and amplification of nucleic acids. However, real time PCR, also known as quantitative PCR (qPCR), allows sensitive and specific quantification of nucleic acids. The product portfolio of Levprot includes enzymes for conventional PCR and real time PCR, but also for reverse-transcription PCR, DNA digestion and ligation reactions, as well as Master Mixes for DNA and RNA samples.

mRNA Synthesis

mRNA synthesis has gained importance since mRNA vaccines and therapeutics became relevant in biopharma. Synthesis of mRNA requires several steps from DNA template to pure mRNA, including post-translational modifications and additional components needed for mRNA synthesis. Levprot offers a product portfolio that includes products that covers all of the above.

Isotermal amplification

Isothermal nucleic acid amplification is a unique method to amplify limited amounts of DNA at constant temperature, eliminating the thermal cycling process and thus, the need of a thermocycler equipment. One of the most common isothermal amplification methods is LAMP (loop-mediated isothermal amplification), in which loop structures facilitate rounds of amplification using 4-6 primers. At Levprot we offer a Bst DNA polymerase which is uniquely useful for a variety of isothermal amplification reactions.

CLIA Enzymes

Chemiluminescent immunoassay (CLIA) is a technique that uses luminescent molecules which  emit electromagnetic radiation (i.e. producing light) triggered by a chemical reaction. This immunoassay technique is more sensitive than conventional colorimetric ELISA methods as it allows the detection of target molecules in biological samples at zeptomole scale (10-21 mol) due to chemiluminescent signal multiplication and amplification.

Blocking buffers

Blocking buffers are inert solutions of a single or a combination of proteins that bind onto surfaces of different nature. These buffers prevent non-specific interactions of antibodies or antigens to membranes or plates, reducing background interferences and increasing the signal-to-noise ratio in immunoassays.

Next generation Sequencing

Next Generation Sequencing (NGS) is a parallel sequencing tech that offers more speed and scalability and is very useful to determine the order of nucleotides in entire genomes of DNA or RNA. NGS has several enzymatic workflow steps, in which polymerases, restriction enzymes, ligases and kinases-phosphorilases have an important role.

Antigens

Antigens are foreign molecules that trigger an immune response acting against what is considered a possible external attack. Their presence makes the immune system produce antibodies against them. The production of antigens is useful for immunoassays as they allow the specific detection of antibodies (i.e. markers of disease) against an antigen.

Enzymes for life sciences

Explore our range of enzymes and specialized products designed to enhance your experiments in molecular biology. A variety of essential enzymes, including DNases, Proteinase K and recombinant BSA, we provide everything you need to optimize your laboratory and ensure precise and reliable results in your research.