mRNA Protein Synthesis
Inorganic pyrophosphatase 1
Inorganic pyrophosphatase 1 (iPP1) catalyzes the hydrolysis of inorganic pyrophosphate to two orthophosphates. iPP1 is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is highly specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if Zn2+ is present. iPP1 has an homodimeric complex, consisting in two equal 32 kDa subunits.
Liquid, in glycerol
*Please, do not hesitate to contact us for further requirements.
According to the purpose, this product is available in its RNase-free version.
*For research use only.
Suggested reaction conditions
Recommended use of 1-3 units per mL of sample in buffer 100 mM Tris pH 7.5, 1-10 mM MgCl2 and 2 mM inorganic pyrophosphatase (iPP) in a high-yield in vitro RNA synthesis reaction.
Liquid versions of this product are provided in buffer 100X concentrated.
Activity unit definition
One unit of the enzyme hydrolysis 1 µmol of inorganic pyrophosphate in 1 min at 25 °C. Enzyme activity is assayed in the following mixture: 100 mM Tris pH 7.5, 2 mM MgCl2 and 2 mM inorganic pyrophosphate (PPi).
Once received, store the product at -80 °C or -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature.
Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -20 °C or -80 °C.
Liquid products are commercialized with 10X Inorganic Pyrophosphatase I reaction buffer, while the lyophilized product only contains the enzyme.
- 01U-G1IPPHDA: 20 mM Tris pH 7,5, 100 mM NaCl, 0,1 mM EDTA, 1 mM DTT
- 01U-S1IPPHDA: 20 mM Tris pH 7.5, 100 mM NaCl, 50% glycerol, 0.1 mM EDTA, 1 mM DTT
- B1IPPHDA (10X concentrated): 1 M Tris pH 7.5, 20 mM MgCl2
Recommended resuspension buffer for the lyophilized products:
- For long-term storage: 20 mM Tris pH 7.5, 100 mM NaCl, 50% glycerol, 0.1 mM EDTA, 1 mM DTT
- For short-term storage: 20 mM Tris pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT
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