PCR Enzymes and Solutions

M-MLV Retro Transcriptase

Moloney Murine Leukemia Virus Reverse TranscripTase (M-MLV RT) is a DNA polymerase using single-strand RNA or DNA as template to synthesize a complementary DNA strand. This enzyme synthesizes the first-strand cDNA up to 7 kb. M-MLV RT contains several point mutations in the RNase H domain that enhances its thermostability and increases the cDNA yield of full-length transcripts (>5 kb) than wild-type M-MLV RT.

Catalog

Reference	Unit size	Concentration	Format	Associated files
MT50U-G1MVRTHA	50,000 U	200 U/µL	Liquid, glycerol-free	TDS SDS
MT50U-L1MVRTHA	50,000 U	N/A**	Lyophilized	TDS SDS
MT50U- S1MVRTHA	50,000 U	200 U/µL	Liquid, in glycerol	TDS SDS

*Please, do not hesitate to contact us for further requirements.

**Not applicable

Applications

Due to the purpose of this product, it is always RNase-free.

*For research use only.

Suggested reaction conditions

Recommended use of 200 units per 20 μL reaction. Mix components template RNA, primers, dNTPs, provided buffer (Ref.: BMVRTHA) and enzyme and incubate at 37 °C for 30 min in a high-yield in vitro RNA synthesis reaction.

Activity unit definition

One unit incorporates 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT)25 as template-primer.

Storage conditions

Once received, store the product at -80 °C or -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature.

Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C.

Buffer composition

Liquid products are commercialized with 10X M-MLV Retro Transcriptase reaction buffer, while the lyophilized product only contains the enzyme.

Product:

- 01U-G1MVRTHA: 20 mM Tris pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Tween-20.
- 01U-S1MVRTHA: 20 mM Tris pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Tween-20, 50% Glycerol

Buffer:

- BMVRTHA (10X): 500 mM Tris pH 8.8, 400 mM KCl, 50 mM MgCl2, 10 mM DTT, 0.1% Tween, 5 mg/mL rBSA

Recommended resuspension buffer for the lyophilized products:

- - For long-term storage: 20 mM Tris pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Tween-20, 50% Glycerol
  - For short-term storage: 20 mM Tris pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Tween-20

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