PCR Enzymes and Solutions
M-MLV Retro Transcriptase
Moloney Murine Leukemia Virus Reverse TranscripTase (M-MLV RT) is a DNA polymerase using single-strand RNA or DNA as template to synthesize a complementary DNA strand. This enzyme synthesizes the first-strand cDNA up to 7 kb. M-MLV RT contains several point mutations in the RNase H domain that enhances its thermostability and increases the cDNA yield of full-length transcripts (>5 kb) than wild-type M-MLV RT.
Liquid, in glycerol
*Please, do not hesitate to contact us for further requirements.
Due to the purpose of this product, it is always RNase-free.
*For research use only.
Suggested reaction conditions
Recommended use of 200 units per 20 μL reaction. Mix components template RNA, primers, dNTPs, provided buffer (Ref.: BMVRTHA) and enzyme and incubate at 37 °C for 30 min in a high-yield in vitro RNA synthesis reaction.
Activity unit definition
One unit incorporates 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT)25 as template-primer.
Once received, store the product at -80 °C or -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature.
Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C.
Liquid products are commercialized with 10X M-MLV Retro Transcriptase reaction buffer, while the lyophilized product only contains the enzyme.
- 01U-G1MVRTHA: 20 mM Tris pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Tween-20.
- 01U-S1MVRTHA: 20 mM Tris pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Tween-20, 50% Glycerol
- BMVRTHA (10X): 500 mM Tris pH 8.8, 400 mM KCl, 50 mM MgCl2, 10 mM DTT, 0.1% Tween, 5 mg/mL rBSA
Recommended resuspension buffer for the lyophilized products:
- For long-term storage: 20 mM Tris pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Tween-20, 50% Glycerol
- For short-term storage: 20 mM Tris pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Tween-20
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