Pharmaceutical and in vitro diagnostic products
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PCR Enzymes and solutions
Polymerase Chain Reaction (PCR) is one of the most common techniques inmolecular biology, having a wide range of applications. Conventional PCR is a qualitative technique for detection and amplification of nucleic acids. However, real time PCR, also known as quantitative PCR (qPCR), allows sensitive and specific quantification of nucleic acids. The product portfolio of Levprot includes enzymes for conventional PCR and real time PCR, but also for reverse-transcription PCR, DNA digestion and ligation reactions, as well as Master Mixes for DNA and RNA samples.
mRNA synthesis has gained importance since mRNA vaccines and therapeutics are becoming relevant in biopharma. Synthesis of mRNA requires several steps from DNA template to pure mRNA, including post-translational modifications and additional components needed for mRNA synthesis. Levprot offers a product portfolio that includes products that covers all of the above.
Isothermal nucleic acid amplification is a unique method to amplify limited amounts of DNA at constant temperature, eliminating the thermal cycling process and thus, the need of a thermocycler equipment. One of the most common isothermal amplification methods is LAMP (loop-mediated isothermal amplification), in which loop structures facilitate rounds of amplification using 4-6 primers.
Next Generation Sequencing
Next Generation Sequencing (NGS) is a parallel sequencing tech that offers more speed and scalability and is very useful to determine the order of nucleotides in entire genomes of DNA or RNA. NGS has several enzymatic workflow steps, in which polymerases, restriction enzymes, ligases and kinases-phosphorilases have an important role.
Chemiluminescent immunoassay (CLIA) is a technique that uses a luminescent molecule which emits electromagnetic radiation caused by a chemical reaction to produce light. This immunoassay technique is more sensitive than conventional colorimetric ELISA methods as it allows to detect targets in biological samples in a zeptomole scale (10-21 mol) due to chemiluminescent signal multiplication and amplification.
CLIA Enzymes (coming soon)
Blocking buffers are inert solutions of a single or a combination of proteins that bind onto surfaces of different nature. These buffers prevent non-specific interactions of antibodies or antigens to membranes or plates, reducing background interferences and increasing the signal-to-noise ratio in immunoassays.
Antigens are considered foreign molecules that prompt the immune system to act against a possible external attack. Antigens induce the immune system to produce antibodies against them. The production of antigens is useful for immunoassays as they allow the detection of antibodies, markers of disease, against an antigen.