PCR Enzymes and Solutions
Taq DNA polymerase
Taq DNA polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. This enzyme catalyses 5’→3’ synthesis of DNA, has no detectable 3’→5’ exonuclease activity and possesses 5’→3’ exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products.
DNA polymerase
Taq DNA polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. This enzyme catalyses 5’→3’ synthesis of DNA, has no detectable 3’→5’ exonuclease activity and possesses 5’→3’ exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products.
Catalog
Applications
- Routine PCR amplification of DNA fragments up to 5 kb
- DNA labelling
- qPCR
Since this Taq DNA polymerase is expressed in yeast, it does not have E. coli DNA contamination, which makes this product more suitable for bacterial DNA analysis than other Taq DNA polymerases expressed in E. coli.
*For research use only.
Suggested reaction conditions
Each 20 μL PCR reaction should carry 2 U of Taq DNA polymerase in provided reaction buffer (BTAQPXA). Reaction buffer is provided 10X concentrated and should be diluted to have a final concentration of 1X (1X buffer contains 10 mM Tris pH 8.3, 50 mM KCl, 0.01% Tween and 1.5 mM MgCl2). Taq DNA polymerase can be used in common PCR conditions.
**Please, see the technical data sheet for more information.
Activity unit definition
One unit of activity is defined as the amount of enzyme needed to incorporate 10 nmol of dNTPs (deoxyribonucleotides) into a DNA fragment, in 30 minutes at 72 °C.
Storage conditions
Once received, store the product at -80 °C or -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature. Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C or -20°C.
Buffer composition
Liquid products are commercialized with 10X Taq DNA polymerase reaction buffer, while the lyophilized product only contains Taq DNA polymerase.
Product:
- 01U-G1TAQPXA: 20 mM Tris pH 7.5, 300 mM KCl, 0.1 mM EDTA, 4% Trehalose, 0.05% Tween, 1 mM DTT (also commercialized as RNase-free product with the following product code: 01U-G1TAQPXA-RF)
- 01U-S1TAQPXA: 20 mM Tris pH 7.5, 300 mM KCl, 0.1 mM EDTA, 0.05% Tween, 1 mM DTT, 50% glycerol (also commercialized as RNase-free product with the following product code: 01U-S1TAQPXA-RF)
Buffer:
- BTAQPXA (10X concentrated): 200 mM Tris pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.1% Tween-20, 10 mM DTT, 5 mg/ml rBSA.
Recommended resuspension buffer for the lyophilized products:
- For long-term storage: 20 mM Tris pH 7.5, 300 mM KCl, 0.1 mM EDTA, 0.05% Tween, 1 mM DTT, 50% glycerol
- For short-term storage: 20 mM Tris pH 7.5, 300 mM KCl, 0.1 mM EDTA, 4% Trehalose, 0.05% Tween, 1 mM DTT
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