Blocking Buffers
Recombinant Bovine Serum Albumin
Recombinant Bovine Serum Albumin (rBSA) is a non-animal albumin, equivalent to the common Bovine Serum Albumin (BSA), although heterelogously expressed in yeast cells. It prevents non-specific adhesion of enzymes to plastic surfaces, and stabilizes proteins during incubation, with even better efficiency than BSA. Moreover, it offers far more consistency and homogeneity than its bovine homologous.
Technical information & Catalog
Illustration of percentage of animal-derived BSA and Levprot’s rBSA necessary to obtain a similar signal in 3 different ELISAs. Set 1 corresponds to a sandwich ELISA, set 2 and 3 corresponds to indirect ELISAs.
Specific signal detected at a fixed concentration of antigen (bars) and non-specific signal detection without any antigen presence (dots).
Catalog
| Cat. No. | Size | Concentration | Format | Special characteristics | Associated files |
| MT01G-G1RBSAHB | 1 g | 100 mg/mL | Liquid, glycerol-free | N/A** | |
| MT01G-L1RBSAHB | 1 g | N/A** | Lyophilized | N/A** | |
| MT01G-S1RBSAHB | 1 g | 100 mg / mL | Liquid, in glycerol | N/A** |
*Please, do not hesitate to contact us for further requirements.
**Not applicable
Applications
rBSA is an ideal blocking solution for various immunoassays, such as:
- ELISA
- Western Blot
- CLIA
- Lateral Flow Assays
- Immunohistochemistry (IHC)
*For research use only.
Suggested reaction conditions
Performance of rBSA as a blocking agent is between 20 and 100 times superior to animal-derived BSA. This means diluting 1:20 to 1:100 of rBSA is recommended when replacing common BSA.
Storage conditions
Once received, store the product at -80 °C or -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots.
Buffer composition
Product:
- 01G-G1RBSAHB: 20 mM Tris pH 7.5, 100 mM KCl, 0.1 mM EDTA
- 01G-G1RBSAHB: 20 mM Tris pH 7.5, 100 mM KCl, 0.1 mM EDTA, 30% glycerol
Recommended resuspension buffer for the lyophilized products:
- For long-term storage: 20 mM Tris pH 7.5, 100 mM KCl, 0.1 mM EDTA, 30% glycerol
- For short-term storage: 20 mM Tris pH 7.5, 100 mM KCl, 0.1 mM EDTA
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Antigens are foreign molecules that trigger an immune response acting against what is considered a possible external attack. Their presence makes the immune system produce antibodies against them. The production of antigens is useful for immunoassays as they allow the specific detection of antibodies (i.e. markers of disease) against an antigen.
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Explore our range of enzymes and specialized products designed to enhance your experiments in molecular biology. A variety of essential enzymes, including DNases, Proteinase K and recombinant BSA, we provide everything you need to optimize your laboratory and ensure precise and reliable results in your research.
