Blocking Buffers

Recombinant Bovine Serum Albumin

Recombinant Bovine Serum Albumin (rBSA) is a non-animal albumin, equivalent to the common Bovine Serum Albumin (BSA), although heterelogously expressed in yeast cells. It prevents non-specific adhesion of enzymes to plastic surfaces, and stabilizes proteins during incubation, with even better efficiency than BSA. Moreover, it offers far more consistency and homogeneity than its bovine homologous. 

Technical information & Catalog

rBSA-elisa-2

Performance of animal-free rBSA blocking ELISA experiments.

rBSA-elisa-step1

Coating surface with polyclonal anybody.

rBSA-elisa-step2

Addition of blocking agent till a final 1% concentration.

rBSA-elisa-step3

Antigen binding to the antibody. Use of blocking agent at 0.1%.

rBSA-elisa-step4

Primary antibody binding to the antigen.

rBSA-elisa-step5

Conjugated antibody binding to the primary antibody. Use of blocking agent at 0.1%.

rBSA-elisa-step6

Signal detection at 450 nm.

Applications

Reference

Size

Concentration

Format

Special characteristics

Associated files

MT10K-G1RBSAHA

10 mg

20 mg/mL

Liquid, glycerol-free

Molecular biology grade

TDS

SDS

MT10K- S1RBSAHA

10 mg

20 mg/mL

Liquid, in glycerol

Molecular biology grade

TDS

SDS

MT10K-L1RBSAHA

10 mg

N/A**

Lyophilized

Molecular biology grade

TDS

SDS

MT01G-G1RBSAHB

1 g

100 mg/mL

Liquid, glycerol-free

N/A**

TDS

SDS

MT01G-L1RBSAHB

1 g

N/A**

Lyophilized

N/A**

TDS

SDS

 

*Please, do not hesitate to contact us for further requirements.  

**Not applicable 

Applications

According to the purpose, this product is also commercialized as a molecular biology grade product. 
*For research use only. 

Suggested reaction conditions

Either rBSA or common BSA perform similarly when used in restriction enzyme digestions. Performance of rBSA as a blocking agent is around 100 times superior to common BSA. This means a dilution 1:100 of rBSA is recommended when replacing common BSA. 

Storage conditions

Once received, store the product at -80 °C or -20 °C freezer, excluding the lyophilized product that can be stored at Room TemperatureStorage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C. . 

Buffer composition

Product: 
    • 10K-G1RBSAHA: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA (molecular biology grade) 
    • 10K-S1RBSAHA: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA, 20% glycerol (molecular biology grade) 
    • 01G-G1RBSAHB: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA 

 

Recommended resuspension buffer for the lyophilized products: 
    • For long-term storage: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA, 50% glycerol  
    • For short-term storage: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA

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