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Blocking Buffers | Enzymes for Life Sciences

Recombinant Bovine Serum Albumin

Recombinant Bovine Serum Albumin (rBSA) is a non-animal albumin, equivalent to the common Bovine Serum Albumin (BSA), although heterelogously expressed in yeast cells. It prevents non-specific adhesion of enzymes to plastic surfaces, and stabilizes proteins during incubation, with even better efficiency than BSA. Moreover, it offers far more consistency and homogeneity than its bovine homologous. 

Technical information & Catalog


Coating surface with polyclonal anybody.


Addition of blocking agent till a final 1% concentration.


Antigen binding to the antibody. Use of blocking agent at 0.1%.


Primary antibody binding to the antigen.


Conjugated antibody binding to the primary antibody. Use of blocking agent at 0.1%.


Signal detection at 450 nm.


Performance of animal-free rBSA blocking ELISA experiments.


Cat. No.SizeConcentrationFormatSpecial characteristicsAssociated files
MT10K-G1RBSAHA10 mg20 mg/mLLiquid, glycerol-freeMolecular biology grade



MT10K- S1RBSAHA10 mg20 mg/mLLiquid, in glycerolMolecular biology grade



MT10K-L1RBSAHA10 mgN/A**LyophilizedMolecular biology grade



MT01G-G1RBSAHB1 g100 mg/mLLiquid, glycerol-freeN/A**



MT01G-L1RBSAHB1 gN/A**LyophilizedN/A**



MT01G-S1RBSAHB1 g100 mg / mLLiquid, in glycerolN/A**



*Please, do not hesitate to contact us for further requirements.  

**Not applicable 


According to the purpose, this product is also commercialized as a molecular biology grade product. 
*For research use only. 

Suggested reaction conditions

Either rBSA or common BSA perform similarly when used in restriction enzyme digestions. Performance of rBSA as a blocking agent is around 100 times superior to common BSA. This means a dilution 1:100 of rBSA is recommended when replacing common BSA. 

Storage conditions

Once received, store the product at -80 °C or -20 °C freezer, excluding the lyophilized product that can be stored at Room TemperatureStorage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C. . 

Buffer composition

    • 10K-G1RBSAHA: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA (molecular biology grade) 
    • 10K-S1RBSAHA: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA, 20% glycerol (molecular biology grade) 
    • 01G-G1RBSAHB: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA 


Recommended resuspension buffer for the lyophilized products: 
    • For long-term storage: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA, 50% glycerol  
    • For short-term storage: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA

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