Recombinant Bovine Serum Albumin
Recombinant Bovine Serum Albumin (rBSA) is a non-animal albumin, equivalent to the common Bovine Serum Albumin (BSA), although heterelogously expressed in yeast cells. It prevents non-specific adhesion of enzymes to plastic surfaces, and stabilizes proteins during incubation, with even better efficiency than BSA. Moreover, it offers far more consistency and homogeneity than its bovine homologous.
Technical information & Catalog
Performance of animal-free rBSA blocking ELISA experiments.
Coating surface with polyclonal anybody.
Addition of blocking agent till a final 1% concentration.
Antigen binding to the antibody. Use of blocking agent at 0.1%.
Primary antibody binding to the antigen.
Conjugated antibody binding to the primary antibody. Use of blocking agent at 0.1%.
Signal detection at 450 nm.
Molecular biology grade
Liquid, in glycerol
Molecular biology grade
Molecular biology grade
*Please, do not hesitate to contact us for further requirements.
According to the purpose, this product is also commercialized as a molecular biology grade product.
*For research use only.
Suggested reaction conditions
Either rBSA or common BSA perform similarly when used in restriction enzyme digestions. Performance of rBSA as a blocking agent is around 100 times superior to common BSA. This means a dilution 1:100 of rBSA is recommended when replacing common BSA.
Once received, store the product at -80 °C or -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C. .
- 10K-G1RBSAHA: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA (molecular biology grade)
- 10K-S1RBSAHA: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA, 20% glycerol (molecular biology grade)
- 01G-G1RBSAHB: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA
Recommended resuspension buffer for the lyophilized products:
- For long-term storage: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA, 50% glycerol
- For short-term storage: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA
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