Enzymes for Life Sciences
Recombinant Bovine Serum Albumin – Molecular Biology Grade
Recombinant Bovine Serum Albumin (rBSA), a non-animal albumin equivalent to the common Bovine Serum Albumin (BSA), is heterologously expressed in yeast cells. Similar to BSA, it has demonstrated efficacy in preventing enzyme adhesion to reaction tubes and pipette surfaces, while also stabilizing certain proteins during incubation, especially relevant for overnight reactions.
Its versatility extends to various applications in molecular biology research such as enzymatic assays and nucleic acid manipulation, where its far more consistency than its bovine analogous enhances experimental outcomes.
Catalog
| Cat. No. | Size | Concentration | Format | Special characteristics | Associated files |
| MT10K-G1RBSAHA | 10 mg | 20 mg/mL | Liquid, glycerol-free | Molecular biology grade | |
| MT10K- S1RBSAHA | 10 mg | 20 mg/mL | Liquid, in glycerol | Molecular biology grade | |
| MT10K-L1RBSAHA | 10 mg | N/A** | Lyophilized | Molecular biology grade |
*Please, do not hesitate to contact us for further requirements.
**Not applicable
Applications
- Prevents adhesion of enzymes to surfaces from reaction tubes or pipette surfaces.
- Keeps the activity of enzymes and proteins in solution.
- Protein stabilization during incubation in enzymatic assays.
- Long-term storage since it stabilizes proteins.
*For research use only.
Suggested reaction conditions
Either rBSA or common BSA perform similarly when used in restriction enzyme digestions or DNA manipulation.
Storage conditions
Once received, store the product at -80 °C or -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots.
Buffer composition
Product:
- 10K-G1RBSAHA: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA (Molecular Biology Grade)
- 10K-S1RBSAHA: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA, 30% glycerol (Molecular Biology Grade)
Recommended resuspension buffer for the lyophilized products:
- For long-term storage: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA, 30% glycerol
- For short-term storage: 20 mM Tris pH 7,5, 100 mM KCl, 0,1 mM EDTA
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