Raw Materials for Life Sciences – PCR & RT Enzymes
M-MLV Reverse Transcriptase
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is a RNA-dependent DNA polymerase used to generate first-strand complementary DNA (cDNA) from poly-mRNA, total RNA or viral RNA for use in downstream applications such as RT-(q)PCR or cDNA cloning. This enzyme contains several point mutations in the RNase H domain that enhances its thermostability and increases the cDNA yield of full-length transcripts (>5 kb) than wild-type M-MLV RT.
Catalog for M-MLV Reverse Transcriptase
Cat. No.
Size
Format
MT50U-L1MVRTHA
50,000 U
Lyophilized
Applications for M-MLV Reverse Transcriptase
- Preparation of cDNA libraries
- First-strand cDNA synthesis
- Quantitative real-time PCR (RT-qPCR)
