Recombinant protein expression and production company
Agricultural & food
The increasing enthusiasm within the agri-food sector has led to the emergence of numerous start-ups and companies harnessing this advanced technology.
Biopharmacy and in vitro diagnostics
At Levprot, we create biopharmacy and in vitro diagnostic products, including enzymes and inhibitory buffers, to support these applications.
Pharmaceutical and in vitro raw materials
PCR Enzymes and solutions
Polymerase chain reaction (PCR) is one of the most common techniques in molecular biology, having a wide range of applications. Conventional PCR is a qualitative technique for detection and amplification of nucleic acids. However, real time PCR, also known as quantitative PCR (qPCR), allows sensitive and specific quantification of nucleic acids. The product portfolio of Levprot includes enzymes for conventional PCR and real time PCR, but also for reverse-transcription PCR, DNA digestion and ligation reactions, as well as Master Mixes for DNA and RNA samples.
mRNA synthesis has gained importance since mRNA vaccines and therapeutics became relevant in biopharma. Synthesis of mRNA requires several steps from DNA template to pure mRNA, including post-translational modifications and additional components needed for mRNA synthesis. Levprot offers a product portfolio that includes products that covers all of the above.
Isothermal nucleic acid amplification is a unique method to amplify limited amounts of DNA at constant temperature, eliminating the thermal cycling process and thus, the need of a thermocycler equipment. One of the most common isothermal amplification methods is LAMP (loop-mediated isothermal amplification), in which loop structures facilitate rounds of amplification using 4-6 primers. At Levprot we offer a Bst DNA polymerase which is uniquely useful for a variety of isothermal amplification reactions.
Next generation Sequencing
Next Generation Sequencing (NGS) is a parallel sequencing tech that offers more speed and scalability and is very useful to determine the order of nucleotides in entire genomes of DNA or RNA. NGS has several enzymatic workflow steps, in which polymerases, restriction enzymes, ligases and kinases-phosphorilases have an important role.
Chemiluminescent immunoassay (CLIA) is a technique that uses luminescent molecules which emit electromagnetic radiation (i.e. producing light) triggered by a chemical reaction. This immunoassay technique is more sensitive than conventional colorimetric ELISA methods as it allows the detection of target molecules in biological samples at zeptomole scale (10-21 mol) due to chemiluminescent signal multiplication and amplification.
Blocking buffers are inert solutions of a single or a combination of proteins that bind onto surfaces of different nature. These buffers prevent non-specific interactions of antibodies or antigens to membranes or plates, reducing background interferences and increasing the signal-to-noise ratio in immunoassays.
Antigens are foreign molecules that trigger an immune response acting against what is considered a possible external attack. Their presence makes the immune system produce antibodies against them. The production of antigens is useful for immunoassays as they allow the specific detection of antibodies (i.e. markers of disease) against an antigen.
At Levprot, we offer recombinant proteins that serve as targets for different antibodies. Check our product portfolio to find the latest pharmaceutical and in vitro diagnostic protein technology.
What is precision fermentation technology?
Precision fermentation aims to produce specific end-products using genetically modified microorganisms such as yeast or bacteria.
Specialists in service of recombinant protein production
At Levprot, we have an extensive experiene in achieving high-yield protein expression in yeast. Our expertise covers all facets of the process, starting from genetic design and extending to the meticulous characterization of the final product. Hence, we provide a comprehensive service covering all stages, accommodating individual steps based on your specific needs. We encourage you to contact us to discuss how we can help you.
Genetic design and strain engineering
Bioprocess development & scale-up
Purification and characterization
Freeze-drying and preservation