SARS-CoV-2 Receptor Binding Domain
A coronavirus contains four structural proteins: spike, envelope, membrane, and nucleocapsid proteins, of which the spike protein plays the most important roles during infection by binding to a host receptor through the receptor-binding domain (RBD) in the S1 subunit and then fusing the viral and host membranes through the S2 subunit. Therefore, the receptor-binding domain serves as a target for development of antibodies, entry inhibitors and vaccines.
*Please, do not hesitate to contact us for further requirements.
*For research use only.
Medium- and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C.
Other recombinant proteins you can buy
PCR Enzymes and solutions
PCR is one of the most common techniques in a wide range of applications. Conventional PCR is a qualitative technique for detection and amplification of nucleic acids. However, real-time PCR, also known as quantitative PCR (qPCR), allows sensitive and specific quantification of nucleic acids. The catalogue of Levprot includes enzymes for conventional PCR and real-time PCR, but also for reverse-transcription PCR, DNA digestion and ligation reactions, as well as Master Mixes for DNA and RNA samples.
mRNA synthesis has gained importance since mRNA vaccines and therapeutics are becoming relevant in biopharma. Synthesis of mRNA requires several steps from DNA template to pure mRNA, including post-translational modifications and additional components needed for mRNA synthesis. The catalogue of Levprot includes products that fulfill these demands.
In isothermal amplification nucleic acids amplification can be executed at constant temperature. Otherwise, PCR needs cyclic temperature changes to work properly. One of the most common isothermal amplification methods is LAMP (loop-mediated isothermal amplification), in which loop structures facilitate rounds of amplification using 4-6 primers.
Chemiluminescent immunoassay (CLIA) is a technique that uses a luminescent molecule which emits electromagnetic radiation caused by a chemical reaction to produce light. This immunoassay technique is more sensitive than conventional colorimetric ELISA methods as it allows to detect targets in biological samples in a zeptomole scale (10-21 mol) due to chemiluminescent signal multiplication and amplification.
Blocking buffers are inert solutions of a single protein or combination of proteins that bind to all remaining surfaces. These buffers prevent non-specific interactions of antibodies or antigens to membranes or plates, reducing background interferences and increasing the signal-to-noise ratio in immunoassays.
Next generation Sequencing
Blocking buffers is a solution of a combination of proteins, or even single protein that absorbs all remaining binding surfaces of the plate. These buffers avoid non-specific interactions of the antibodies or antigens to membranes or plates, reducing background and increasing signal-to-noise ratio in immunoassays.
Antigens are considered foreign molecules that prompt the immune system to act against a possible external attack. Antigens induce the immune system to produce antibodies against them. The production of antigens is useful for immunoassays as they allow the detection of antibodies, markers of disease, against an antigen.
At Levprot, we offer recombinant proteins that serve as targets for different antibodies. Check our catalog to find the latest pharmaceutical and in vitro diagnostic protein technology.