Enzymes for Life Sciences
Proteinase K
Proteinase K is a broad-spectrum serine protease enzyme, isolated from the fungus Tritirachium album. It efficiently digests proteins by cleaving peptide bonds adjacent to the carboxylic group of aromatic and aliphatic amino acids. Due to its stability in the presence of denaturing agents, chelating reagents and in a wide range of pH (4-12.5) and the ability to digest native or denatured proteins, proteinase K is suitable for removing DNases and RNases.
Catalog
| Cat. No. | Unit size | Concentration | Format | Special characteristics | Associated files |
| MT20K-S1PRTKXA | 20 mg | ~20 mg/mL* | Liquid, with glycerol | N/A** | Coming soon. |
| MT20K-L1PRTKXA | 20 mg | N/A** | Lyophilized | N/A** | Coming soon. |
| MT1CK-S1PRTKXA | 100 mg | ~20 mg/mL* | Liquid, with glycerol | N/A** | Coming soon. |
| MT1CK-L1PRTKXA | 100 mg | N/A** | Lyophilized | N/A** | Coming soon. |
*~20 mg/mL corresponds to >600 U/mL
**Not applicable
Please, do not hesitate to contact us for further requirements.
Applications
- Removal of DNases and RNases during DNA or RNA isolation from cell lines or tissues
- Removal of associated proteins in a nucleic acid purification
- Determination of enzyme localization
- Removal of nucleases for in situ hybridization
- Enhancement of cloning efficiency of PCR products
Suggested reaction conditions
Recommended use of 0.05-1 mg of Proteinase K per mL of sample. Incubate the mix at 55 °C for 15 min and, if necessary, inactivate the enzyme at 95 °C for 10 min. Enzyme activity can be enhanced by SDS and urea.
Storage conditions
Once received, store the product at -80 °C o -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature.
Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C.
Buffer composition
- 20K-S1PRTKXA and 1CK-S1PRTKXA: 20mg/ml in 20mM Tris pH 7.5, 3 mM CaCl2 and 50% glycerol.
Recommended resuspension buffer for the lyophilized products:
- For long-term storage: 20 mM Tris pH 7.5, 3 mM CaCl2 and 50% glycerol
- For short-term storage: 20 mM Tris pH 7.5, 3 mM CaCl2
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Next generation Sequencing
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Enzymes for life sciences
Explore our range of enzymes and specialized products designed to enhance your experiments in molecular biology. A variety of essential enzymes, including DNases, Proteinase K and recombinant BSA, we provide everything you need to optimize your laboratory and ensure precise and reliable results in your research.
