Enzymes for Life Sciences

Proteinase K

Proteinase K is a broad-spectrum serine protease enzyme, isolated from the fungus Tritirachium album. It efficiently digests proteins by cleaving peptide bonds adjacent to the carboxylic group of aromatic and aliphatic amino acids. Due to its stability in the presence of denaturing agents, chelating reagents and in a wide range of pH (4-12.5) and the ability to digest native or denatured proteins, proteinase K is suitable for removing DNases and RNases.

Catalog

Reference Unit size  Concentration Format Special characteristics Associated files 
MT20K-S1PRTKXA20 mg~20 mg/mL* Liquid, with glycerolN/A** 

Coming soon.

MT20K-L1PRTKXA20 mgN/A** LyophilizedN/A** Coming soon.
MT1CK-S1PRTKXA100 mg~20 mg/mL* Liquid, with glycerolN/A** Coming soon.
MT1CK-L1PRTKXA100 mgN/A** LyophilizedN/A** Coming soon.

*~20 mg/mL corresponds to >600 U/mL

**Not applicable

Please, do not hesitate to contact us for further requirements.

Applications

Suggested reaction conditions

Recommended use of 0.05-1 mg of Proteinase K per mL of sample. Incubate the mix at 55 °C for 15 min and, if necessary, inactivate the enzyme at 95 °C for 10 min. Enzyme activity can be enhanced by SDS and urea.

Storage conditions

Once received, store the product at -80 °C o -20 °C freezer, excluding the lyophilized product that can be stored at Room Temperature. 

Medium and long-term storage from -20 °C to -80 °C. Storage at 4 °C is possible for short term. Avoid multiple freeze/thaw cycles by storing multiple aliquots at -80 °C.

Buffer composition

    • 20K-S1PRTKXA and 1CK-S1PRTKXA: 20mg/ml in 20mM Tris pH 7.5, 3 mM CaCl2 and 50% glycerol.
  •  
 Recommended resuspension buffer for the lyophilized products: 
    • For long-term storage: 20 mM Tris pH 7.5, 3 mM CaCl2 and 50% glycerol
    • For short-term storage: 20 mM Tris pH 7.5, 3 mM CaCl2

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