Next Generation Sequencing

DNA Polymerase δ

DNA Polymerase δ is a DNA-dependent polymerase complex found in eukaryotic cells. In eukaryotes, this polymerase has a major role in genome maintenance since it shows a very high fidelity and processivity. In-vitro, it presents a misincorporation rate of 0.3 to 1 base substitutions per 100,000 nucleotides and thanks to its 3’ à 5’ exonuclease activity, the base substitution can be further decreased.  

Applications

Since this DNA Polymerase δ is expressed in yeast, it does not have E. coli DNA contamination, which makes this product more suitable for bacterial DNA analysis than other DNA polymerases expressed in E. coli. 

*For research use only. 

Suggested reaction conditions

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Activity unit definition

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Storage conditions

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Next generation Sequencing

Next Generation Sequencing (NGS) is a parallel sequencing tech that offers more speed and scalability and is very useful to determine the order of nucleotides in entire genomes of DNA or RNA. NGS has several enzymatic workflow steps, in which polymerases, restriction enzymes, ligases and kinases-phosphorilases have an important role.

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