Next Generation Sequencing
DNA Polymerase δ
DNA Polymerase δ is a DNA-dependent polymerase complex found in eukaryotic cells. In eukaryotes, this polymerase has a major role in genome maintenance since it shows a very high fidelity and processivity. In-vitro, it presents a misincorporation rate of 0.3 to 1 base substitutions per 100,000 nucleotides and thanks to its 3’ à 5’ exonuclease activity, the base substitution can be further decreased.
Applications
- Next-generation sequencing (NGS)
- Large DNA amplification
- High fidelity amplification
Since this DNA Polymerase δ is expressed in yeast, it does not have E. coli DNA contamination, which makes this product more suitable for bacterial DNA analysis than other DNA polymerases expressed in E. coli.
*For research use only.
Suggested reaction conditions
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Activity unit definition
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Storage conditions
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